PathoSEEK® Salmonella & (STEC) E. coli Multiplex Detection Assay uses a multiplexing strategy with an internal plant DNA reaction control to ensure accurate detection of Salmonella & (STEC) E. coli as well as cannabis DNA in every reaction. Unlike other techniques, this multiplexing strategy verifies the performance of the assay when detecting pathogens, resulting in the minimization of false negative results due to reaction set-up errors or failing experimental conditions. Below is a simplified depiction of the qPCR assays. The use of magnetic particles affords 8 or 96 tip automation, enabling high throughput applications. DNA can be isolated from a single sample or a large batch in under 1 hour. Hands-on time is less than 45 minutes.